Terminator
Part:BBa_K2934003:Design
Designed by: Nir Litver Group: iGEM19_Technion-Israel (2019-10-10)
AmyE 3' UTR from Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a short part of the 5'-UTR of the gene, which includes the terminator sequence and several bases upstream and downstream to the terminator.
Source
The sequence was taken from SubtiWiki site [1], and the terminator determined using ARNold tool [2].
References
[1] http://www.subtiwiki.uni-goettingen.de/v3/genome/?gene=DE4857E8A228F145EFB9B7AD817585258344ED1F
[2] http://rssf.i2bc.paris-saclay.fr/toolbox/arnold/