Terminator

Part:BBa_K2934003:Design

Designed by: Nir Litver   Group: iGEM19_Technion-Israel   (2019-10-10)


AmyE 3' UTR from Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a short part of the 5'-UTR of the gene, which includes the terminator sequence and several bases upstream and downstream to the terminator.


Source

The sequence was taken from SubtiWiki site [1], and the terminator determined using ARNold tool [2].

References

[1] http://www.subtiwiki.uni-goettingen.de/v3/genome/?gene=DE4857E8A228F145EFB9B7AD817585258344ED1F

[2] http://rssf.i2bc.paris-saclay.fr/toolbox/arnold/